Engineering the substrate specificity of xylose isomerase
نویسندگان
چکیده
منابع مشابه
Engineering the substrate specificity of xylose isomerase.
Xylose isomerase (XI) catalyzes the isomerization and epimerization of hexoses, pentoses and tetroses. In order to clarify the reasons for the low reaction efficiency of a pentose sugar, L-arabinose, we determined the crystal structure of Streptomyces rubiginosus XI complexed with L-arabinose. The crystal structure revealed that, when compared with D-xylose and D-glucose, L-arabinose binds to t...
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Xylose utilization is one key issue for the bioconversion of lignocelluloses. It is a promising approach to engineering heterologous pathway for xylose utilization in Saccharomyces cerevisiae. Here, we constructed a xylose-fermenting yeast SyBE001 through combinatorial fine-tuning the expression of XylA and endogenous XKS1. Additional overexpression of genes RKI1, RPE1, TKL1, and TAL1 in the no...
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5 Sun-Mi Lee, Taylor Jellison, and Hal S. Alper* 6 7 8 1 Department of Chemical Engineering, The University of Texas at Austin,1 University Station, 9 C0400,Austin, Texas 78712 10 11 2 Clean Energy Research Center, Korea Institute of Science and Technology, 39-1 Hawolgok12 dong, Seongbuk-gu, Seoul 136-791, Korea 13 14 3 Institute for Cellular and Molecular Biology, The University of Texas at Au...
متن کاملCrystallographic studies of D-xylose isomerase.
* This work was supported by National Institutes of Health Grants CA-10925, CA-OG927, and RR-05539 from the United States Public Health Service and by an appropriation from the Commonwealth of Pennsylvania. (I), was crystallized from acetone. The enzyme from Bacillus coagulans has also been crystallized from ammonium sulfate solution and from aqueous acetone (3). We describe here preliminary x-...
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BACKGROUND Yeasts tolerant to toxic inhibitors from steam-pretreated lignocellulose with xylose co-fermentation capability represent an appealing approach for 2nd generation ethanol production. Whereas rational engineering, mutagenesis and evolutionary engineering are established techniques for either improved xylose utilisation or enhancing yeast tolerance, this report focuses on the simultane...
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ژورنال
عنوان ژورنال: Protein Engineering Design and Selection
سال: 2005
ISSN: 1741-0126,1741-0134
DOI: 10.1093/protein/gzh099